Projects & Collaborations

ABC scientists collaborate with colleagues in academia and industry from around the world to better understand oyster biology and enhance oyster aquaculture. This effort involves both traditional histology as well as new, cutting-edge tools and techniques including near-infrared reflectance spectrometry, cytogenetics, and probiotics.

Consortium

Shellfish geneticists from 12 East Coast universities will work together on a $4.4 million dollar grant funded by NOAA Fisheries through the Atlantic States Marine Fisheries Commission to develop new tools for studying the oyster genome. Gene sequencing technology will allow researchers at ABC to more accurately predict breeding values associated with various traits, thereby increasing the rate of improvement in the trait of interest. Other researchers will use this technology to look for specific genes associated with traits such as low salinity tolerance or resistance to ocean acidification. Learn more.

Hatchery Health (HAT)

The Hatchery Health (HAT) project is a collaborative investigation by more than 20 specialists from VIMS, VA Sea Grant, and Virginia Tech. This project explores strategies for managing water chemistry and their effect on successful hatchery production. ABC helped investigate how different water treatments affect larval health and the microbiome of hatchery water. Researchers took clean seawater from 2 different filtration stages, as well as water from healthy oyster broodstock tanks and used it to inoculate larvae tanks. Then, they monitored growth and survival of the larvae as well as chemical water quality.

Post-doc Eric Guévélou grinds freeze-dried oyster tissue into a powder that will be used for near-infrared reflectance spectrometry.

Near-infrared reflectance spectrometry (NIRS)

Led by Dr. Eric Guévélou, near-infrared reflectance spectrometry (NIRS) allows ABC to learn about the chemical composition of an oyster by immersing a probe in oyster tissues. When the data from the reflected light is calibrated into a model, the results reveal information about glycogen, lipid, and water composition, all of which can have important implications on the overall health of the oyster. Learn more. 

 
 

 

Post-doc Joana Sousa uses a microscope to perform cytogenetic analysis of oyster larvae cells. This technique allows her to count the exact number of chromosomes in a single cell.
Cytogenetics

Led by Dr. Joana Sousa, cytogenetics research allows ABC to better understand the loss of chromosomes from polyploid oysters. Chromosome instability from polyploid oysters is of major scientific interest and of practical concern for commercial oyster culture. Until now, flow cytometry (FCM) was ABC’s principal research tool for detecting chromosome loss, however, there is little information in FCM data about the exact number of chromosomes that are missing. With cytogenetic techniques, ABC is able to examine the chromosomes themselves, to contribute to the developing body of knowledge about the heritability of chromosome instability in polyploid oysters. Learn more.

URI Probiotics

Can oyster larvae benefit from probiotic bacteria like humans do? It appears so. Several research groups around the country have been investigating this topic and working to develop probiotic formulations for bivalve hatcheries. These could help outcompete or eliminate bacterial pathogens that are commonly found in bivalve hatcheries and can cause devastating disease outbreaks in larval cultures. Dr. Marta Gomez-Chiarri’s group at the University of Rhode Island has been working with two strains of probiotic bacteria that have been proven to reduce pathogens in laboratory challenges, without any negative affect on larval health. Since 2013, ABC has collaborated on several projects with this group to test these probiotic strains at a larger scale. By examining different formulations and delivery methods within the hatchery setting, researchers hope to develop successful products that will be commercially available to aquaculture facilities around the country and will be able reduce unpredictable and devastating economic losses at these sites. Learn more.