Data descriptors for the 100 lobster project.

 

General lobster dissection and data (Prof. Jeffrey Shields)

ri_id:

Identification numbers for the lobsters. ME represents lobsters from Maine, RI represents lobsters from Rhode Island.

Location:

Lobsters from Rhode Island were all caught under the Newport Bridge in the East Passage. It is roughly 4 nm south of Prudence.

Study:

(Intentionally blank)

Date received:

Date received at VIMS. (ancillary data on necropsy date and individual performing the necropsy are available separately)

CL:

Carapace length from the orbit to the rear of the carapace, in mm. (Conversion to inches by dividing by 25.4).

Injury:

Number of injuries on an animal. These do not include the occasional artifactual injuries associated with shipment and handling such as recent autotomy of a limb.

Dead:

0 if dead at time of necropsy, 1 if alive at time of necropsy.

Sex:

M = male, F = female

Disease:

Presence of epizootic shell disease; 0 = no shell disease present, 1 = shell disease present.

Intensity:

Disease intensity based on previous papers (0 = no disease, light = <10% of the carapace affected, moderate = 11-50% of the carapace affected, heavy = >50% of the carapace affected).

Observed molt:

Molt stage determined by shell hardness and examination of a gill cleaner.

Molt Status:

Molt stage determined via histology.

TCBS:

Thiosulfate citrate bile sucrose agar is relatively specific for bacterial species of Vibrio. Data are presence of vibrio colonies grown from the hemolymph of a lobster (neg = negative, pos = colonies present).

 

Metals assessments (Prof. Lawrence LeBlanc)

Status:

same as Disease descriptor above.

Intensity:

same as intensity descriptor above.

Molt_stage:

Observed molt as above.

Sex:

Same as Sex above.

Fraction (dry weight):

Dry weight of the tissue fraction used to assess elements in the hepatopancreas of lobsters.

As_dry:

Arsenic as mg/Kg dry weight

B_dry:

Boron as mg/Kg dry weight

Cd_dry:

Cadmium as mg/Kg dry weight

Co_dry:

Cobalt as mg/Kg dry weight

Cr_dry:

Chromium as mg/Kg dry weight

Cs_dry:

Cesium as mg/Kg dry weight

Mn_dry:

Manganese as mg/Kg dry weight

Mo_dry:

Molybdenum as mg/Kg dry weight

Ni_dry:

Nickel as mg/Kg dry weight

Pb_dry:

Lead as mg/Kg dry weight

V_dry:

Vanadium as mg/Kg dry weight

Ca_dry:

Calcium as mg/Kg dry weight

Cu_dry:

Copper as mg/Kg dry weight

Fe_dry:

Iron as mg/Kg dry weight

K_dry:

Potassium as mg/Kg dry weight

Mg_dry:

Magnesium as mg/Kg dry weight

S_dry:

Sulfur as mg/Kg dry weight

Ba_dry:

Barium as mg/Kg dry weight

Li_dry:

 Lithium as mg/Kg dry weight

Sb_dry:

Antimony as mg/Kg dry weight

Sr_dry:

Strontium as mg/Kg dry weight

Ti_dry:

Titanium as mg/Kg dry weight

U_dry:

as mg/Kg dry weight

As_wet:

Arsenic as mg/Kg wet weight

B_wet:

Boron as mg/Kg wet weight

Cd_wet:

Cadmium as mg/Kg wet weight

Co_wet:

Cobalt as mg/Kg wet weight

Cr_wet:

Chromium as mg/Kg wet weight

Cs_wet:

Cesium as mg/Kg wet weight

Mn_wet:

Manganese as mg/Kg wet weight

Mo_wet:

Molybdenum as mg/Kg wet weight

Ni_wet:

Nickel as mg/Kg wet weight

Pb_wet:

Lead as mg/Kg wet weight

V_wet:

Vanadium as mg/Kg wet weight

Ca_wet:

Calcium as mg/Kg wet weight

Cu_wet:

Copper as mg/Kg wet weight

Fe_wet:

Iron as mg/Kg wet weight

K_wet:

Potassium as mg/Kg wet weight

Mg_wet:

Magnesium as mg/Kg wet weight

S_wet:

Sulfur as mg/Kg wet weight

Ba_wet:

Barium as mg/Kg wet weight

Li_wet:

Lithium as mg/Kg wet weight

Sb_wet:

Antimony as mg/Kg wet weight

Sr_wet:

Strontium as mg/Kg wet weight

Ti_wet:

Titanium as mg/Kg wet weight

U_wet:

as mg/Kg wet weight

 

Histology (Dr. Roxana Smolowitz)

carapace formation stage :

histological assessment of the molt stage of the cuticle.

# carapace pieces on the slide:

1-5 pieces of cuticle mounted on a histological slide for evaluation.

piece #:

Individual pieces were assigned a number for reference in the Lesion_characters data.

lesion prev/piece:

prevalence of lesions on each piece. Single numbers indicate that the lesion prevalence was the same on each piece. Multiple numbers indicate the number of lesions present on respective pieces.

Lesion severity:

severity of each lesion present on each piece. Single numbers indicate that the severity of the lesion was the same on each piece. Multiple numbers indicate the severity of lesions on respective pieces. See also Lesion_characters.

RI Cell present/development?:

presence of reserve inclusion cells in the underlying epidermis and their relative size. These cells can sequester glycogen which is used in the production of chitin for the development of the cuticle.

tegmental glands pesent/development:

presence of tegmental glands and their relative number. These glands are involved in molting. They secrete lubricants that allow the animal to molt properly. They also function in maintaining the cuticle in the intermolt animal.

Presence of pillars in the lesion:

y = cuticular pillars present in the lesion, n = pillars not present. The pillars are a hallmark of epizootic shell disease.

photos:

photographs of the histology were taken. NB: these photos have not been included in the 100 lobster data set at this time.

Lesion_characters:

Descriptive notes characterizing the lesions in the cuticle. Piece or section numbers refer to the piece # above.